Restoration of bone structure using allogeneic hydroxyapatite Lioplast in animals with experimental osteorezorbtsii

Restoration of bone structure using allogeneic HAP in animals with experimental osteoreorption.

INJECTION METHOD FOR INJECTION OF HYDROXYAPATITIS.

Ectopic implantation in the femoral muscle (40 mg).

Depot "allogeneic hydroxyapatite" in the muscle. first day of introduction.

Scanning electron microscopy. Magnification: 30.

TECHNOLOGY FOR PRODUCING ALLOGENOUS HYDROXYAPATITIS.

ALLOGENIC HYDROXYAPATITIS (HAP)

CHEMICAL COMPOSITION

 

• Inorganic component

 

Ca - 413-537 mg / g

P -167-380 mg / g

Mg -1.30-3.50 mg / g

Fe - 0.09-0.026 mg / g

Zn - 0.01-0.820 mg / g

Co - 0,011-0,024 mg / g

Cr - 0.006-0.020 mg / g

Ag - 0.009-0.02 mg / g

• Organic component

Chondroitin sulfate - 0.08-0.140 mg / g

Collagen - 336.5 mg / g

ELECTRONIC MICROSCOPY OF ALLOGENIC HYDROXYAPATITIS

Scanning probe microscopy

3D image of a porous surface with GAP particles

Transmission microscopy

Nanoparticle "allogeneic hydroxyapatite". TEM × 165120

PURPOSE OF THE STUDY

At the preclinical stage, using morphological methods, determine the degree of effectiveness and safety of the injection method of using allogenic HAP for the prevention and treatment of osteoporosis.

PRECLINICAL TESTS

IN VITRO CELL CULTURES

 

RESEARCH METHODS

• Morphological
• Biochemical

- Electron microscopy

IN VIVO ANIMALS

Hydroxyapatitis
The crystals of the mineral component of bone tissue - "Allogenic hydroxyapatite" series "Lyoplast" ® pH> 8

Pronounced proliferation, restoration of monolayer density. Stimulation of collagenogenesis.

Dermal fibroblasts 1 day. Inverted microscope. Magnification: 100

Dermal fibroblasts 4 days. Inverted microscope. Magnification: 100

DEPOT OF ALLOGENIC HAP IN MUSCLE TISSUE

RASTER ELECTRONIC MICROSCOPY

1 day after administration

10 days after administration

MUSCLE TISSUE IN THE ALLOGENOUS HAP INJECTION AREA

MUSCLE TISSUE IN THE INJECTION AREA WITHOUT CHANGES

Coloring by van gieson. Magnification: 100

DISSEMINATION OF ALLOGENIC HAP

Coloring by Van Koss. Magnification: 100

HYPESTROGENIC MODEL OF OSTEOPOROSIS

BILATERAL OVARIOECTOMY 2.5 MONTHS

Color: Van Gieson. Magnification: 100

1 - destruction of bone beams;

2 - thinning of bone beams;

3 - resorption niche with an osteoclast.

Color: hematoxylin and eosin. Magnification: 400

PREVENTION OF OSTEORESORPTION

the introduction of allogenic HAP on the day of surgery

Color: hematoxylin and eosin. Increase: 100

Bone tissue remodeling

  HAP INTRODUCTION IN 1 MONTH AFTER OVARIOEKTOMY

2.5 months from the start of the experiment

 

1 - active osteoblasts;

2 - newly formed bone tissue.

Coloring: Hematoxylin and eosin. Magnification: 400

NO PATHOLOGICAL CHANGES

OF THE INTERNAL RAT ORGANS AT THE INTRODUCTION OF ALLOGENIC HAP

Drug kidney. Coloring: CHIC reaction according to Mac Manus. Magnification: 400

 

Spleen preparation. Color: hematoxylin and eosin. Magnification: 40

ABSENCE OF PATHOLOGICAL CHANGES AND CALCIFICATES

AT THE INTRODUCTION OF ALLOGENIC HAP

Lung tissue preparation. Color: hematoxylin and eosin. Magnification: 400

Lung tissue preparation. Color: Van Cossu. Magnification: 400